prof. dr hab. Moraczewska JoannaEnglish

prof. dr hab. Moraczewska JoannaEnglish

CURRICULUM VITAE

 

Positions

 

-      Since 2009, Associate Professor, Head of the Department of Biochemistry and Cell Biology

-      Since April 2014, Vice-Dean for Scientific Matters

 

Education and degrees

 

-        1987 - M.Sc., Biology/Molecular biology, Warsaw University, Poland

-        1993 -  Ph.D., Biochemistry, Nencki Institute of Experimental Biology in Warsaw, Polish Academy of Science

-        2008 - D.Sc., Biochemistry, Nencki Institute of Experimental Biology in Warsaw, Polish Academy of Science

 

Positions and scientific experience

 

-        1987-1993, research assistant, Nencki Institute of Experimental Biology in Warsaw

-        1990, 1991, visiting scientist, Medical University in Pecs, Central Laboratory, Pecs, Hungary

-        1990, visiting scientist, Institute of Cytology, Russian Academy of Science, Sankt Petersburg, Russia

-        1993, visiting scientist, Sydney University, Dept. of Anatomy Muscle Research Unit, Sydney, Australia

-        1994-1997, Adjunct researcher, Nencki Institute of Experimental Biology in Warsaw

-        1997-2000, post-doctoral fellow, University of Medicine and Dentistry of New Jersey, Piscataway, USA

-        2000-2008, Assistant professor, Kazimierz Wielki University in Bydgoszcz

-        2004-2008, The Wellcome Trust fellow

-        2009-present, Associate professor, Kazimierz Wielki University in Bydgoszcz

 

Scientific interests

 

-        thin filament regulation in muscle and non-muscle cells

-        role of tropomyosin isoforms in functional diversification of actin filaments

-        tropomyosin elasticity as a molecular mechanism of actin filament regulation

-        molecular mechanisms of actin filament dynamics regulation by muscle and non-muscle isoforms of cofilin

-        cooperative and allosteric transmission of functional conformational changes in microfilaments

-        effects of myopathy-causing missense mutations in tropomyosin and cofilin on the thin filament functions.

 

Experimental techniques:

 

-    muscle proteins isolation and purification

-   expression and purification of recombinant proteins

-   site-directed mutagenesis

-   enzymatic assays (actin and myosin ATPase activity)

-   protein labeling and chemical modifications

-   limited proteolysis

-   steady state fluorescence

-   fluorescence anisotropy

-   Forster Resonance Energy Transfer (FRET)

-   in vitro motility assay

-   Western Blot

-   co-sedimentation assay.

 

Professional memberships


Polish Biochemical Society, Member of the Executive Board.

 

Scientific sessions and conference organization

 

-        Congress of Polish Biochemistry and Cell Biology, Kraków 2011, Member of Scientific Committee, Chair of international session on “Molecular mechanisms of cell motility”

-    48th Annual Meeting of Polish Biochemical Society, Toruń 2013, Chair of Scientific Committee and Chair of session on “Cellular motility in pathology”

-    European Muscle Conference, Warsaw 2015, Member of the Organizing Committee and Chair of session on “Acto-myosin interactions“


Reviews for:

 

-        Acta Biochimica Polonica

 Biochimica et Biophysica Acta

-        Postępy Biochemii

-        Biophysical Journal

-        Cytoskeleton

-        Biochimica et Biophysica Acta

-        Peer Journal (scientific editor)

-        FEBS Journal

-        Journal of Biological Chemistry


Teaching experience


-        Lecturer, instructor and examiner in several courses (Biochemistry, Biochemistry of proteins and nucleic acids, Bioinorganic chemistry, Techniques in biochemistry, Techniques in fluorescence, Molecular mechanisms of cellular motility) for undergraduate and graduate students.

-        Supervisor of experimental diploma work of graduate students (master degree)

-        Supervisor of PhD students

 

Awards


-        1990 – Minister of Education award for publication "Biochemical and theoretical approach to localization of metal-ion-binding sites in the actin primary structure", Eur. J. Biochem. 182, 299-305 (1989) Eur. J. Biochem. 211, 731-748

-        1994- Parnas Award of Polish Biochemical Society for the best experimental work done in Polish laboratory in 1993 for publication "Localization of the tightly bound divalent-cation-dependent and nucleotide-dependent conformation changes in G-actin using limited proteolytic digestion"

-        1998 - American Heart Association Award in form of stipend for studies on structural determinants in tropomyosin in cooperative regulation of actin-myosin interactions

-        2005 – Scientific Award of Biological Faculty of Polish Academy of Science for co-authoring publications on structural bases of motility generated by motor protein myosin and actin

-        2008 – Award of Rector of Kazimierz Wielki University for scientific achievements

-        2010 – Award of Kazimierz Wielki University Rector for scientific achievements

 

Non-scientific interests and leisure activities

-        Mountain hiking, cycling and swimming

-        Opera and classical music

-        Fiction reading

 

LIST of PUBLICATIONS

 

Original experimental work:

 

  1. Jerzmanowski, A., Moraczewska, J. (1987) A charged contaminant prevents Physarum chromatin from aggregating. Biochim.Biophys.Acta 910, 111-115.
  2. Jerzmanowski, A., Moraczewska, J. (1988) Distribution of postsynthetic methylation sites in Physarum histone H1. Mol.Biol.Rep.13, 97-101.
  3. Strzelecka-Gołaszewska, H., Boguta, G., Zmorzyński, S., Moraczewska, J. (1989) Biochemical and theoretical approach to localization of metal-ion-binding sites in the actin primary sites in the actin primary structure. Eur.J.Biochem. 182, 299-305.
  4. Strzelecka-Gołaszewska, H., Moraczewska, J., Khaitlina, S.Y., Mossakowska, M. (1993) Localization of the tightly bound divalent-cation-dependent and nucleotide-dependent conformational changes in G-actin using limited proteolytic digestion. Eur.J.Biochem. 211, 731-748.
  5. Khaitlina, S.Y., Moraczewska, J., Strzelecka-Gołaszewska, H. (1993) The actin-actin interactions involving the N-terminus portion of the DNaseI-binding-loop are crucial for stabilization of the actin filament. Eur.J.Bioch. 218, 911-920.
  6. Mossakowska, M., Moraczewska, J., Khaitlina, S.Y., Strzelecka-Gołaszewska, H. (1993) Proteolytic removal of three C-terminal residues of actin alters the monomer-monomer interactions. Biochem.J. 289, 897-902.
  7. Strzelecka-Gołaszewska, H., Mossakowska, M., Woźniak, A., Moraczewska, J., Nakayama, H. (1995) Long-range conformational effects of proteolytic removal of the last three residues of actin. Biochem.J. 307, 527-534.
  8. Moraczewska, J., Strzelecka-Gołaszewska, H., Moens, P.,D.,J., dos Remedios, C.G. (1996) Structural changes in subdomain 2 of G-actin observed by fluorescence spectroscopy. Biochem.J. 317, 605-611.
  9. Moraczewska, J., Wawro, B., Seguro, K., Strzelecka-Gołaszewska, H. (1999) Divalent  Cation-, and Polymerization-Dependent Changes in the Conformation of Subdomain 2 of Actin. Biophys.J. 77, 373-377.
  10. Moraczewska, J., Nicholson-Flynn, K., Hitchocock-DeGregori, S.E. (1999) The Ends of Tropomyosin Are Major Determinants of Actin Affinity and Myosin Subfragment 1-Induced Binding to F-Actin in the Open State. Biochemistry 38, 15885-15892.
  11. Moraczewska, J., Hitchcock-DeGregori, S.E. (2000) Independent Functions for the N- and C-termini in the Overlap Region of Tropomyosin. Biochemistry 39, 6891-6897.
  12. Borovikov Y.S., Moraczewska J., Khoroshev M.I., Strzelecka-Gołaszewska H. (2000) Proteolytic Cleavage of Actin Within the DNase-I-Binding Loop Changes the Conformation of F-actin and Its Sensitivity to Myosin Binding. Biochim. Biophys. Acta 1478, 138-151.
  13. Moraczewska, J., Greenfield, N.J., Liu, Y., Hitchcock-DeGregori, S.E. (2000) Alteration of Tropomyosin Function and Folding by a Nemaline Myopathy-Causing Mutation. Biophys. J. 79, 3217-3225.
  14. Hitchcock-DeGregori, S. E., Song, Y., Moraczewska, J. (2001) Importance of Internal Regions and the Overall Length of Tropomyosin for Actin Binding and Regulatory Function. Biochemistry 40, 2104-2112.
  15. Moraczewska, J., Gruszczyńska-Biegała, J., Rędowicz M.J., Khaitlina, S., Strzelecka-Gołaszewska, H. (2004) The Dnase-I Binding Loop May Play a Role in the Regulation of Actin-Myosin Interaction by Tropomyopsin/Troponin. J.Biol.Chem. 279, 31197-31204.
  16. Kłopocka ,W., Moraczewska, J., Rędowicz, M.J. (2005) Characterization of Rac/PAK pathway in Amoeba proteus. Protoplasma, 225, 77-84.
  17. Śliwińska M., Skórzewski R., Moraczewska J. (2008) Role of Actin C Terminus in Regulation of Striated Muscle Thin Filament. Biophys. J.94, 1341-1347.
  18. Skórzewski, R., Śliwińska, M., Borys, D., Sobieszek, A., Moraczewska J. (2009) Effect of actin C-terminal modification on tropomyosin isoforms binding and thin filament regulation. Biochim.Biophys. Acta – Prot. Proteom. 1794, 237-243.
  19. Skórzewski R, Robaszkiewicz K, Jarzębińska J, Suder P, Silberring J, Moraczewska J. (2009) Differential binding of tropomyosin isoforms to actin modified with m-maleimidobenzoyl-N-hydroxysuccinimide ester and fluorescein-5-isothiocyanate. Anal Biochem. 1; 394: 48-55.
  20. Śliwińska M., Żukowska M., Borys D., Moraczewska J. (2011) Different positions of tropomyosin on actin filament are determined by specific overlaps of end-to-end overlaps. Cytoskeleton 68, 300-312.
  21. Robaszkiewicz K., Dudek E., Kasprzak, A.A., Moraczewska J. (2012) Functional effects of congenital myopathy related mutations in gamma-tropomyosin gene. Biochim.Biophys.Acta - Mol. Basis Dis.1822, 1562-1569.
  22. Jurewicz E., Ostrowska Z., Jóźwiak J., Rędowicz M.J., Leśniak W., Moraczewska J., Filipek A. (2013) CacyBP/SIP as a novel modulator of the thin filament. Biochim. Biophys. Acta – Mol. Cell Res. 1833, 761-766.
  23. Jędrzejewska B, Bajorek A, Moraczewska J. (2013) Interaction of carbocyanine dyes with DNA: synthesis and spectroscopic studies. Appl. Spectrosc. 67 (6):672-80.
  24. Śliwińska M, Moraczewska J. (2013) Structural differences between C-terminal regions of tropomyosin isoforms. PeerJ. Oct 15;1:e181.
  25. Robaszkiewicz K., Ostrowska Z., Cyranka-Czaja A., Moraczewska J. (2015) Impaired tropomyosin-troponin interactions reduce activation of the actin thin filament Biochim.Biophys. Acta – Prot. Proteom.1854, 381-390.
  26. Robaszkiewicz K, Ostrowska Z, Marchlewicz K, Moraczewska J. (2016) Tropomyosin isoforms differentially modulate the regulation of actin filament polymerization and depolymerization by cofilins. FEBS J. 283(4):723-737.
  27. Borovikov YS, Rysev NA, Chernev AA, Avrova SV, Karpicheva OE, Borys D, Śliwińska M, Moraczewska J. (2016) Abnormal movement of tropomyosin and response of myosin heads and actin during the ATPase cycle caused by the Arg167His, Arg167Gly and Lys168Glu mutations in TPM1 gene. Arch Biochem Biophys. 606:157-166.
  28. Ostrowska Z, Robaszkiewicz K, Moraczewska J. (2017) Regulation of actin filament turnover by cofilin-1 and cytoplasmic tropomyosin isoforms. Biochim Biophys Acta – Prot. Proteom. 1865: 88-98
  29. Borovikov YS, Rysev NA, Avrova SV, Karpicheva OE, Borys D, Moraczewska J. Molecular mechanisms of deregulation of the thin filament associated with the R167H and K168E substitutions in tropomyosin Tpm1.1. Arch Biochem Biophys. 2016 Dec 9. pii: S0003-9861(16)30557-4.(doi: 10.1016/j.abb.2016.12.004).

 

Review articles:

 

  1. Moraczewska, J. (2001) Tropomyosin – a regulatory protein of  thin filament Kosmos, 50, 236-270. (in Polish)
  2. Moraczewska, J. (2002) Structural determinants of cooperativity in acto-myosin interactions. Acta Biochim.Pol. 49 (4), 805-812.
  3. Moraczewska J. (2009) The role of tropomyosin isoforms in diversification of actin filaments functions. Post. Bioch. 55: 201-206. (in Polish)
  4. Robaszkiewicz K., Moraczewska J. (2011) congenital myopathy-related mutations in gamma-tropomyosin gene. . Post.Hig.Med.Dośw., 65, 347-356. (in Polish)
  5. Moraczewska J. Śliwińska M., Rędowicz M.J. (2012) Calcium ions in the regulation of acto-myosin interactions. Post. Bioch. 58, .437-451. (in Polish)
  6. Rędowicz MJ, Moraczewska J. (2015) Insight into muscle physiology through understanding mechanisms of muscle pathology. J Muscle Res Cell Motil. 36(6):359-361
  7. Ostrowska Z., Moraczewska J. (2016) Cofilin- the regulator of actin filament dynamics Post.Hig.Med.Dośw., in press. (in Polish)

 

 

Conference abstracts (last 10 years):

  1. Moraczewska J. (2005) Tropomyosin flexibility and its importance for thin filament regulation. Folia Histochem. Cytobiol. 43 (Suppl.1) 131.
  2. Moraczewska J., Skórzewski R., Śliwińska M. Borys M. (2006) Involvement of Actin C-terminus in Regulation of Actomyosin ATPase by Tropomyosin Isoforms. Biophys. J. Abstract Issue.
  3. Moraczewska J., Piątek K., Skórzewski R., Borys D. (2006) Interactions of Tropomyosin Isoforms with Actin Thin Filament. J. Muscle Res. Cell Motil.  27 (5-7), 483.
  4. Moraczewska J. (2006) Effect of actin modification on the regulation of actin-myosin interactions by muscle and nonmuscle isoforms of tropomyosin. Acta Biochim. Pol. 53 (Suppl. 1) 33.
  5. Śliwńska M., Skórzewski R., Moraczewska J. (2006) Tropomyosin-actin contact sites. Acta Biochim. Pol. 53 (Suppl. 1) 34.
  6. Moraczewska J. (2006) Functional Importance of Actin Molecular Dynamics. The Wellcome Trust Senior Fellows Meeting, London, UK, Abstract Book.
  7. Moraczewska J., Śliwińska M. (2007) Different Interactions of Tropomyosin Isoforms with Actin Thin Filament. International Conference on Molecular Recognition. Pecs, Hungary, Abstract Book.
  8. Moraczewska J. (2008) Mechanisms of actin filament regulation by tropomyosins. Acta Bioch. Pol. 55 (Suppl. 3), 82.
  9. Robaszkiewicz K., Skórzewski R., Moraczewska J. (2008) Modification of actin subdomain 2 differentially affects thin filament regulation by tropomyosin isoforms. Acta Biochim. Pol. 55 (Suppl 3), 31.
  10. Skórzewski R., Robaszkiewicz K., Moraczewska J. (2008) Localization of MBS incorporation sites in monomeric actin. J. Muscle Res. Cell Motil. 28(608), 259.
  11. Moraczewska J., Śliwińska M., Żukowska M., Borys D. (2009) Differential myosin-induced movement of tropomyosin isoforms on actin filament. Biophys. J. Abstract Issue.
  12. Śliwińska M., Moraczewska J. (2010) Flexibility of Tropomyosin Isoforms Measured by Förster Resonance Energy Transfer. Acta Biochim. Pol. 57 (Suppl 4), 44.
  13. Robaszkiewicz K., Moraczewska J. (2010) Effects of Tropomyosin Mutations Related to Congenital Fibre Type Disproportion on the Regulation of Actin Filament Acta Biochim. Pol. 57 (Suppl 4), 52.
  14. Kasprzak A., Moraczewska J., Robaszkiewicz K., Kocik E.(2011) Effects of myopathy causing mutations in tropomyosin Arg167 on the thin filament assembly and regulation. (European Muscle Conference, Berlin) J. Muscle Res. Cell Motil. 32, 362.
  15. Śliwińska M., Moraczewska J. (2011) Isoform-specific structural changes of tropomyosin ends during actin filament activation. (European Muscle Conference, Berlin) J. Muscle Res. Cell Motil. 32, 364.
  16. Jurewicz E., Ostrowska Z., Jóźwiak J., Rędowicz J, Moraczewska J., Filipek A. (2012) The influence of the CacyBP/SIP protein on the actin-tropomyosin complex. Acta Biochim. Pol. 59 (Suppl 3).
  17. Robaszkiewicz K., Ostrowska Z., Moraczewska J. (2013) Tropomyosin mutations related to human congenital myopathies change tropomyosin interactions with troponin. Acta Biochim. Pol. 60 (Suppl 1).
  18. Śliwińska M., Moraczewska J (2014) Tropomyosin region encoded by alternative exon 2 confers different flexibility to smooth muscle and non-muscle isoforms. (FEBS Meeting, Paris) FEBS J. 281 (Suppl. 1), 782.
  19. Robaszkiewicz K., Ostrowska Z., Moraczewska J (2014) Regulation of actin dynamics by non-muscle tropomyosin and cofilin isoforms. FEBS Congress, Paris. FEBS J. 281 (Suppl. 1), 780.
  20. Ostrowska Z., Robaszkiewicz K., Krupińska K., Moraczewska J.(2014) Regulation of contractile and cytoskeletal actin filaments by cofilin. Acta Biochim. Pol. 61 (Suppl 1).
  21. Moraczewska J., Cyranka-Czaja A., Robaszkiewicz K., Ostrowska Z. (2014) Effects of Congenital Myopathy Mutations in Tropomyosin on Troponin Binding to the Thin Filament. (European Muscle Conference, Salzburg) . J. Muscle Res Cell Motil. 36, 78.
  22. Simonyan A., Borovikov Y., Robaszkiewicz K., Borys D., Moraczewska J. (2015) The effects of α-tropomyosin Arg245Gly and Glu241Leu mutants on the structural states of actomyosin during the ATPase cycle. (FEBS Congress, Berlin). FEBS J. 282 Suppl. 1, 333.
  23. Rysev N.A., Karpicheva O.E., Avrova S.V., Robaszkiewicz K., Borys D., Borovikov Y.S., Moraczewska J. (2015) The Arg167His and Lys168Glu mutations in a-tropomyosin (Tpm1.1) disrupt the regulation of actin-myosin interaction during the ATPase cycle.(European Muscle Conference, Warsaw) J. Muscle Res. Cell Motil. 36, 538.
  24. Robaszkiewicz K., Ostrowska Z., Moraczewska J.  (2015) The effects of cofilin and non-muscle tropomyosin isoforms Tpm3.2 and Tpm3.4 on the regulation of actin filaments dynamics. .(European Muscle Conference, Warsaw) J. Muscle Res Cell Motil. 36, 577.
  25. Ostrowska Z., Moraczewska J. (2016) Modulation of cofilin severing activity by tropomyosin isoforms. (Bio2016 Congress, Wrocław) Acta Biochim Pol. Suppl 2, 181.
  26. Śliwińska M., Robaszkiewicz K., Moraczewska J. (2016) Effects of R90P and E240K substitutions in tropomyosin Tpm1.1 on the thin filament regulation. (Bio2016 Congress, Wrocław) Acta Biochim Pol. Suppl 2, 178.
  27. Simonyan A., Sirenko V., Robaszkiewicz K., Borys D., Moraczewska J., Krutetskaya Z., Borovikov Y. (2016) Substitutions E240K and R244G in tropomyosin have similar effects on actin-myosin interaction during ATPase cycle. European Muscle Conference, Montpellier, France, Abstract Book. 
  28. Śliwińska M., Robaszkiewicz K., Moraczewska J. (2016) Cardiomyopathy-causing mutations Ile92Thr and Val95Ala in TPM1 have opposite effects on tropomyosin functions. European Muscle Conference, Montpellier, France, Abstract Book.

 

 

prof. dr hab. Moraczewska Joanna